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agonists yoda1  (MedChemExpress)


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    Structured Review

    MedChemExpress agonists yoda1
    Agonists Yoda1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/agonists+yoda1/GsMTx4/pmc13084373-57-3-13
    Average 97 stars, based on 108 article reviews
    agonists yoda1 - by Bioz Stars, 2026-09
    97/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion
    Article Snippet: Fluorescent secondary antibody was purchased from Xi'an Zhuangzhi Biotechnology Co. (China).Fluorescent secondary antibody was purchased from Xi'an Zhuangzhi Biotechnology Co. (China).. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China).

    Article Title: Piezo1/ ITGB1 Synergizes With Ca 2+ / YAP Signaling to Propel Bladder Carcinoma Progression via a Stiffness‐Dependent Positive Feedback Loop
    Article Snippet: The CCK‐8 reagent was sourced from TargetMol (USA).The CCK‐8 reagent was sourced from TargetMol (USA).. Agonists (Yoda1) and inhibitors (GsMTx4) for Piezo1 were acquired from MedChemExpress (China).. The small interfering RNA (si‐RNA) against Piezo1 and ITGB1 was synthesized by Genepharma Technology.The small interfering RNA (si‐RNA) against Piezo1 and ITGB1 was synthesized by Genepharma Technology.

    Article Title: Enhanced Tumor‐Targeted Delivery of Arginine‐Rich Peptides via a Positive Feedback Loop Orchestrated by Piezo1/integrin β1 Signaling Axis
    Article Snippet: Chlorpromazine (CPZ), Methyl‐β‐cyclodextrin (MβCD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK‐8 reagent were purchased from TargetMol (USA).Chlorpromazine (CPZ), Methyl‐β‐cyclodextrin (MβCD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK‐8 reagent were purchased from TargetMol (USA).. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China).. The integrin β1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).The integrin β1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).

    Article Title: Enhanced Tumor-Targeted Delivery of Arginine-Rich Peptides via a Positive Feedback Loop Orchestrated by Piezo1/integrin β1 Signaling Axis.
    Article Snippet: (M𝛽CD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK-8 reagent were purchased from TargetMol (USA). .. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China). .. The integrin 𝛽1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).

    Plasmid Preparation:

    Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion
    Article Snippet: Fluorescent secondary antibody was purchased from Xi'an Zhuangzhi Biotechnology Co. (China).Fluorescent secondary antibody was purchased from Xi'an Zhuangzhi Biotechnology Co. (China).. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China).

    Article Title: Piezo1/ ITGB1 Synergizes With Ca 2+ / YAP Signaling to Propel Bladder Carcinoma Progression via a Stiffness‐Dependent Positive Feedback Loop
    Article Snippet: The CCK‐8 reagent was sourced from TargetMol (USA).The CCK‐8 reagent was sourced from TargetMol (USA).. Agonists (Yoda1) and inhibitors (GsMTx4) for Piezo1 were acquired from MedChemExpress (China).. The small interfering RNA (si‐RNA) against Piezo1 and ITGB1 was synthesized by Genepharma Technology.The small interfering RNA (si‐RNA) against Piezo1 and ITGB1 was synthesized by Genepharma Technology.

    Article Title: Enhanced Tumor‐Targeted Delivery of Arginine‐Rich Peptides via a Positive Feedback Loop Orchestrated by Piezo1/integrin β1 Signaling Axis
    Article Snippet: Chlorpromazine (CPZ), Methyl‐β‐cyclodextrin (MβCD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK‐8 reagent were purchased from TargetMol (USA).Chlorpromazine (CPZ), Methyl‐β‐cyclodextrin (MβCD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK‐8 reagent were purchased from TargetMol (USA).. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China).. The integrin β1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).The integrin β1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).

    Article Title: Enhanced Tumor-Targeted Delivery of Arginine-Rich Peptides via a Positive Feedback Loop Orchestrated by Piezo1/integrin β1 Signaling Axis.
    Article Snippet: (M𝛽CD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK-8 reagent were purchased from TargetMol (USA). .. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China). .. The integrin 𝛽1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).

    Flow Cytometry:

    Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion
    Article Snippet: Fluorescent secondary antibody was purchased from Xi'an Zhuangzhi Biotechnology Co. (China).Fluorescent secondary antibody was purchased from Xi'an Zhuangzhi Biotechnology Co. (China).. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China).

    Article Title: Piezo1/ ITGB1 Synergizes With Ca 2+ / YAP Signaling to Propel Bladder Carcinoma Progression via a Stiffness‐Dependent Positive Feedback Loop
    Article Snippet: The CCK‐8 reagent was sourced from TargetMol (USA).The CCK‐8 reagent was sourced from TargetMol (USA).. Agonists (Yoda1) and inhibitors (GsMTx4) for Piezo1 were acquired from MedChemExpress (China).. The small interfering RNA (si‐RNA) against Piezo1 and ITGB1 was synthesized by Genepharma Technology.The small interfering RNA (si‐RNA) against Piezo1 and ITGB1 was synthesized by Genepharma Technology.

    Article Title: Enhanced Tumor‐Targeted Delivery of Arginine‐Rich Peptides via a Positive Feedback Loop Orchestrated by Piezo1/integrin β1 Signaling Axis
    Article Snippet: Chlorpromazine (CPZ), Methyl‐β‐cyclodextrin (MβCD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK‐8 reagent were purchased from TargetMol (USA).Chlorpromazine (CPZ), Methyl‐β‐cyclodextrin (MβCD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK‐8 reagent were purchased from TargetMol (USA).. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China).. The integrin β1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).The integrin β1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).

    Article Title: Enhanced Tumor-Targeted Delivery of Arginine-Rich Peptides via a Positive Feedback Loop Orchestrated by Piezo1/integrin β1 Signaling Axis.
    Article Snippet: (M𝛽CD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK-8 reagent were purchased from TargetMol (USA). .. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China). .. The integrin 𝛽1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).

    Immunofluorescence:

    Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion
    Article Snippet: Fluorescent secondary antibody was purchased from Xi'an Zhuangzhi Biotechnology Co. (China).Fluorescent secondary antibody was purchased from Xi'an Zhuangzhi Biotechnology Co. (China).. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China).

    Article Title: Piezo1/ ITGB1 Synergizes With Ca 2+ / YAP Signaling to Propel Bladder Carcinoma Progression via a Stiffness‐Dependent Positive Feedback Loop
    Article Snippet: The CCK‐8 reagent was sourced from TargetMol (USA).The CCK‐8 reagent was sourced from TargetMol (USA).. Agonists (Yoda1) and inhibitors (GsMTx4) for Piezo1 were acquired from MedChemExpress (China).. The small interfering RNA (si‐RNA) against Piezo1 and ITGB1 was synthesized by Genepharma Technology.The small interfering RNA (si‐RNA) against Piezo1 and ITGB1 was synthesized by Genepharma Technology.

    Article Title: Enhanced Tumor‐Targeted Delivery of Arginine‐Rich Peptides via a Positive Feedback Loop Orchestrated by Piezo1/integrin β1 Signaling Axis
    Article Snippet: Chlorpromazine (CPZ), Methyl‐β‐cyclodextrin (MβCD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK‐8 reagent were purchased from TargetMol (USA).Chlorpromazine (CPZ), Methyl‐β‐cyclodextrin (MβCD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK‐8 reagent were purchased from TargetMol (USA).. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China).. The integrin β1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).The integrin β1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).

    Article Title: Enhanced Tumor-Targeted Delivery of Arginine-Rich Peptides via a Positive Feedback Loop Orchestrated by Piezo1/integrin β1 Signaling Axis.
    Article Snippet: (M𝛽CD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK-8 reagent were purchased from TargetMol (USA). .. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China). .. The integrin 𝛽1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).

    Activation Assay:

    Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion
    Article Snippet: Fluorescent secondary antibody was purchased from Xi'an Zhuangzhi Biotechnology Co. (China).Fluorescent secondary antibody was purchased from Xi'an Zhuangzhi Biotechnology Co. (China).. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China).

    Article Title: Piezo1/ ITGB1 Synergizes With Ca 2+ / YAP Signaling to Propel Bladder Carcinoma Progression via a Stiffness‐Dependent Positive Feedback Loop
    Article Snippet: The CCK‐8 reagent was sourced from TargetMol (USA).The CCK‐8 reagent was sourced from TargetMol (USA).. Agonists (Yoda1) and inhibitors (GsMTx4) for Piezo1 were acquired from MedChemExpress (China).. The small interfering RNA (si‐RNA) against Piezo1 and ITGB1 was synthesized by Genepharma Technology.The small interfering RNA (si‐RNA) against Piezo1 and ITGB1 was synthesized by Genepharma Technology.

    Article Title: Enhanced Tumor‐Targeted Delivery of Arginine‐Rich Peptides via a Positive Feedback Loop Orchestrated by Piezo1/integrin β1 Signaling Axis
    Article Snippet: Chlorpromazine (CPZ), Methyl‐β‐cyclodextrin (MβCD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK‐8 reagent were purchased from TargetMol (USA).Chlorpromazine (CPZ), Methyl‐β‐cyclodextrin (MβCD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK‐8 reagent were purchased from TargetMol (USA).. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China).. The integrin β1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).The integrin β1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).

    Article Title: Enhanced Tumor-Targeted Delivery of Arginine-Rich Peptides via a Positive Feedback Loop Orchestrated by Piezo1/integrin β1 Signaling Axis.
    Article Snippet: (M𝛽CD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK-8 reagent were purchased from TargetMol (USA). .. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China). .. The integrin 𝛽1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).

    Expressing:

    Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion
    Article Snippet: Fluorescent secondary antibody was purchased from Xi'an Zhuangzhi Biotechnology Co. (China).Fluorescent secondary antibody was purchased from Xi'an Zhuangzhi Biotechnology Co. (China).. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China).

    Article Title: Piezo1/ ITGB1 Synergizes With Ca 2+ / YAP Signaling to Propel Bladder Carcinoma Progression via a Stiffness‐Dependent Positive Feedback Loop
    Article Snippet: The CCK‐8 reagent was sourced from TargetMol (USA).The CCK‐8 reagent was sourced from TargetMol (USA).. Agonists (Yoda1) and inhibitors (GsMTx4) for Piezo1 were acquired from MedChemExpress (China).. The small interfering RNA (si‐RNA) against Piezo1 and ITGB1 was synthesized by Genepharma Technology.The small interfering RNA (si‐RNA) against Piezo1 and ITGB1 was synthesized by Genepharma Technology.

    Article Title: Enhanced Tumor‐Targeted Delivery of Arginine‐Rich Peptides via a Positive Feedback Loop Orchestrated by Piezo1/integrin β1 Signaling Axis
    Article Snippet: Chlorpromazine (CPZ), Methyl‐β‐cyclodextrin (MβCD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK‐8 reagent were purchased from TargetMol (USA).Chlorpromazine (CPZ), Methyl‐β‐cyclodextrin (MβCD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK‐8 reagent were purchased from TargetMol (USA).. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China).. The integrin β1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).The integrin β1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).

    Article Title: Enhanced Tumor-Targeted Delivery of Arginine-Rich Peptides via a Positive Feedback Loop Orchestrated by Piezo1/integrin β1 Signaling Axis.
    Article Snippet: (M𝛽CD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK-8 reagent were purchased from TargetMol (USA). .. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China). .. The integrin 𝛽1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).

    Western Blot:

    Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion
    Article Snippet: Fluorescent secondary antibody was purchased from Xi'an Zhuangzhi Biotechnology Co. (China).Fluorescent secondary antibody was purchased from Xi'an Zhuangzhi Biotechnology Co. (China).. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China).

    Article Title: Piezo1/ ITGB1 Synergizes With Ca 2+ / YAP Signaling to Propel Bladder Carcinoma Progression via a Stiffness‐Dependent Positive Feedback Loop
    Article Snippet: The CCK‐8 reagent was sourced from TargetMol (USA).The CCK‐8 reagent was sourced from TargetMol (USA).. Agonists (Yoda1) and inhibitors (GsMTx4) for Piezo1 were acquired from MedChemExpress (China).. The small interfering RNA (si‐RNA) against Piezo1 and ITGB1 was synthesized by Genepharma Technology.The small interfering RNA (si‐RNA) against Piezo1 and ITGB1 was synthesized by Genepharma Technology.

    Article Title: Enhanced Tumor‐Targeted Delivery of Arginine‐Rich Peptides via a Positive Feedback Loop Orchestrated by Piezo1/integrin β1 Signaling Axis
    Article Snippet: Chlorpromazine (CPZ), Methyl‐β‐cyclodextrin (MβCD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK‐8 reagent were purchased from TargetMol (USA).Chlorpromazine (CPZ), Methyl‐β‐cyclodextrin (MβCD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK‐8 reagent were purchased from TargetMol (USA).. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China).. The integrin β1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).The integrin β1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).

    Article Title: Enhanced Tumor-Targeted Delivery of Arginine-Rich Peptides via a Positive Feedback Loop Orchestrated by Piezo1/integrin β1 Signaling Axis.
    Article Snippet: (M𝛽CD), Dynasore, Cycloheximide (CHX), MG132, Primaquine, Chloroquine (CQ) and CCK-8 reagent were purchased from TargetMol (USA). .. The agonists (Yoda1) and inhibitors (GsMTx4) of PIEZO1 and EIPA were purchased from MedChemExpress (China). .. The integrin 𝛽1 plasmid and PIEZO1 overexpressed plasmid was synthesized by Biokeeper (China).



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    Selleck Chemicals piezo1 agonist yoda1
    <t>Piezo1</t> mediates the suppression of osteogenic gene expression under low‐strain CTS. The knockdown efficiency was confirmed to be approximately 80% for each gene. (A) RT‐qPCR showed that si Piezo1 reversed the low‐strain CTS‐induced downregulation of Runx2 and Osx . (B, C) si Piezo2 or si Trpv4 did not reverse osteogenic gene suppression by low‐strain CTS. An ordinary one‐way analysis of variance (ANOVA), followed by Tukey's multiple comparisons test was used. Each group was compared with the siN/ C without the CTS group. Statistical significance was set at P < 0.05, with significance levels denoted as follows: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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    Image Search Results


    Piezo1 mediates the suppression of osteogenic gene expression under low‐strain CTS. The knockdown efficiency was confirmed to be approximately 80% for each gene. (A) RT‐qPCR showed that si Piezo1 reversed the low‐strain CTS‐induced downregulation of Runx2 and Osx . (B, C) si Piezo2 or si Trpv4 did not reverse osteogenic gene suppression by low‐strain CTS. An ordinary one‐way analysis of variance (ANOVA), followed by Tukey's multiple comparisons test was used. Each group was compared with the siN/ C without the CTS group. Statistical significance was set at P < 0.05, with significance levels denoted as follows: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: JOR Spine

    Article Title: Mechanosensitive Ion Channel PIEZO1 Suppresses BMP2 ‐Induced Ossification of the Annulus Fibrosus Cells

    doi: 10.1002/jsp2.70168

    Figure Lengend Snippet: Piezo1 mediates the suppression of osteogenic gene expression under low‐strain CTS. The knockdown efficiency was confirmed to be approximately 80% for each gene. (A) RT‐qPCR showed that si Piezo1 reversed the low‐strain CTS‐induced downregulation of Runx2 and Osx . (B, C) si Piezo2 or si Trpv4 did not reverse osteogenic gene suppression by low‐strain CTS. An ordinary one‐way analysis of variance (ANOVA), followed by Tukey's multiple comparisons test was used. Each group was compared with the siN/ C without the CTS group. Statistical significance was set at P < 0.05, with significance levels denoted as follows: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: We measured intracellular calcium responses using the calcium indicator dye Fluo‐8 NW (AAT Bioquest, Pleasanton, CA, Cat. #36315) and PIEZO1 agonist Yoda1 (Selleck, Houston, TX, Cat. #S6678).

    Techniques: Gene Expression, Knockdown, Quantitative RT-PCR

    Activation of Piezo1 by Yoda1 suppresses osteogenic markers in rat AF cells. (A) RT‐qPCR showed downregulation of Runx2 , Osx , and Alp after treatment with 10 μM Yoda1 for 12 h. (B) Western blotting analysis revealed that RUNX2 protein levels decreased in rat AF cells after 24‐h Yoda1 treatment. Figure shows the full‐length blot images. Statistical significance was set at P < 0.05, with significance levels denoted as follows: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: JOR Spine

    Article Title: Mechanosensitive Ion Channel PIEZO1 Suppresses BMP2 ‐Induced Ossification of the Annulus Fibrosus Cells

    doi: 10.1002/jsp2.70168

    Figure Lengend Snippet: Activation of Piezo1 by Yoda1 suppresses osteogenic markers in rat AF cells. (A) RT‐qPCR showed downregulation of Runx2 , Osx , and Alp after treatment with 10 μM Yoda1 for 12 h. (B) Western blotting analysis revealed that RUNX2 protein levels decreased in rat AF cells after 24‐h Yoda1 treatment. Figure shows the full‐length blot images. Statistical significance was set at P < 0.05, with significance levels denoted as follows: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: We measured intracellular calcium responses using the calcium indicator dye Fluo‐8 NW (AAT Bioquest, Pleasanton, CA, Cat. #36315) and PIEZO1 agonist Yoda1 (Selleck, Houston, TX, Cat. #S6678).

    Techniques: Activation Assay, Quantitative RT-PCR, Western Blot

    Activation of Piezo1 by Yoda1 suppresses osteogenic markers in human AF cells. Human AF cells were isolated from nondegenerated (Pfirrmann grade 1) and severely degenerated (Pfirrmann grade 5) intervertebral discs. (A) PIEZO1 mRNA expression showed no significant difference between grade 1 and grade 5 cells. (B–E) Consistent with observations in rat AF cells, treatment with Yoda1 (10 μM, 12 h) significantly downregulated osteogenesis‐related genes ( RUNX2 , OSX ) at the mRNA level and reduced RUNX2 protein expression. Full‐length Western blot images are shown in Figure . Statistical significance was set at P < 0.05, with significance levels denoted as follows: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: JOR Spine

    Article Title: Mechanosensitive Ion Channel PIEZO1 Suppresses BMP2 ‐Induced Ossification of the Annulus Fibrosus Cells

    doi: 10.1002/jsp2.70168

    Figure Lengend Snippet: Activation of Piezo1 by Yoda1 suppresses osteogenic markers in human AF cells. Human AF cells were isolated from nondegenerated (Pfirrmann grade 1) and severely degenerated (Pfirrmann grade 5) intervertebral discs. (A) PIEZO1 mRNA expression showed no significant difference between grade 1 and grade 5 cells. (B–E) Consistent with observations in rat AF cells, treatment with Yoda1 (10 μM, 12 h) significantly downregulated osteogenesis‐related genes ( RUNX2 , OSX ) at the mRNA level and reduced RUNX2 protein expression. Full‐length Western blot images are shown in Figure . Statistical significance was set at P < 0.05, with significance levels denoted as follows: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: We measured intracellular calcium responses using the calcium indicator dye Fluo‐8 NW (AAT Bioquest, Pleasanton, CA, Cat. #36315) and PIEZO1 agonist Yoda1 (Selleck, Houston, TX, Cat. #S6678).

    Techniques: Activation Assay, Isolation, Expressing, Western Blot

    Transcriptomic analysis of AF cells following Piezo1 activation by Yoda1. (A) PCA showed a clear separation between the control (DMSO) and Yoda1‐treated groups. (B) Volcano plot of DEGs shows 3281 upregulated and 3362 downregulated genes. (C) GSEA reveals enrichment of ossification‐ and calcium signaling‐related pathways, including regulation of ossification, regulation of bone mineralization, and calcineurin‐mediated signaling, in Yoda1‐treated AF cells.

    Journal: JOR Spine

    Article Title: Mechanosensitive Ion Channel PIEZO1 Suppresses BMP2 ‐Induced Ossification of the Annulus Fibrosus Cells

    doi: 10.1002/jsp2.70168

    Figure Lengend Snippet: Transcriptomic analysis of AF cells following Piezo1 activation by Yoda1. (A) PCA showed a clear separation between the control (DMSO) and Yoda1‐treated groups. (B) Volcano plot of DEGs shows 3281 upregulated and 3362 downregulated genes. (C) GSEA reveals enrichment of ossification‐ and calcium signaling‐related pathways, including regulation of ossification, regulation of bone mineralization, and calcineurin‐mediated signaling, in Yoda1‐treated AF cells.

    Article Snippet: We measured intracellular calcium responses using the calcium indicator dye Fluo‐8 NW (AAT Bioquest, Pleasanton, CA, Cat. #36315) and PIEZO1 agonist Yoda1 (Selleck, Houston, TX, Cat. #S6678).

    Techniques: Activation Assay, Control

    Piezo1 activation inhibits BMP2‐induced osteogenesis through calcineurin signaling. (A) Schematic of the experimental schedule for the BMP2 and Yoda1 treatment. (B) RT‐qPCR showed that Yoda1 cotreatment suppressed the BMP2‐induced upregulation of Osx and Alp in AF cells. (C, D) Alizarin Red staining revealed that BMP2 alone enhanced calcium deposition, whereas cotreatment with Yoda1 reduced this staining intensity. (E) Calcineurin phosphatase assay showing that Yoda1 significantly increased calcineurin enzymatic activity compared with DMSO control; this effect was abolished by co‐treatment with cyclosporin A (CsA). (F, G) Immunocytochemistry for p‐Smad1/5/9 showed increased nuclear translocation after BMP2 treatment but significantly decreased BMP2 + Yoda1 cotreated group. Furthermore, cotreatment with the calcineurin inhibitor CsA rescues the nuclear translocation of p‐Smad1/5/9. Statistical significance was set at P < 0.05, with significance levels denoted as follows: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: JOR Spine

    Article Title: Mechanosensitive Ion Channel PIEZO1 Suppresses BMP2 ‐Induced Ossification of the Annulus Fibrosus Cells

    doi: 10.1002/jsp2.70168

    Figure Lengend Snippet: Piezo1 activation inhibits BMP2‐induced osteogenesis through calcineurin signaling. (A) Schematic of the experimental schedule for the BMP2 and Yoda1 treatment. (B) RT‐qPCR showed that Yoda1 cotreatment suppressed the BMP2‐induced upregulation of Osx and Alp in AF cells. (C, D) Alizarin Red staining revealed that BMP2 alone enhanced calcium deposition, whereas cotreatment with Yoda1 reduced this staining intensity. (E) Calcineurin phosphatase assay showing that Yoda1 significantly increased calcineurin enzymatic activity compared with DMSO control; this effect was abolished by co‐treatment with cyclosporin A (CsA). (F, G) Immunocytochemistry for p‐Smad1/5/9 showed increased nuclear translocation after BMP2 treatment but significantly decreased BMP2 + Yoda1 cotreated group. Furthermore, cotreatment with the calcineurin inhibitor CsA rescues the nuclear translocation of p‐Smad1/5/9. Statistical significance was set at P < 0.05, with significance levels denoted as follows: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: We measured intracellular calcium responses using the calcium indicator dye Fluo‐8 NW (AAT Bioquest, Pleasanton, CA, Cat. #36315) and PIEZO1 agonist Yoda1 (Selleck, Houston, TX, Cat. #S6678).

    Techniques: Activation Assay, Quantitative RT-PCR, Staining, Phosphatase Assay, Activity Assay, Control, Immunocytochemistry, Translocation Assay

    Proposed mechanism schema. Piezo1‐mediated calcium influx activates calcineurin, which dephosphorylates p‐Smad1/5/9, inhibiting its nuclear translocation and BMP2 signaling.

    Journal: JOR Spine

    Article Title: Mechanosensitive Ion Channel PIEZO1 Suppresses BMP2 ‐Induced Ossification of the Annulus Fibrosus Cells

    doi: 10.1002/jsp2.70168

    Figure Lengend Snippet: Proposed mechanism schema. Piezo1‐mediated calcium influx activates calcineurin, which dephosphorylates p‐Smad1/5/9, inhibiting its nuclear translocation and BMP2 signaling.

    Article Snippet: We measured intracellular calcium responses using the calcium indicator dye Fluo‐8 NW (AAT Bioquest, Pleasanton, CA, Cat. #36315) and PIEZO1 agonist Yoda1 (Selleck, Houston, TX, Cat. #S6678).

    Techniques: Translocation Assay